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Fig. 2 | Journal of Ovarian Research

Fig. 2

From: Evaluation of mitochondria in mouse oocytes following cisplatin exposure

Fig. 2

Membrane potential of mitochondria in small immature oocytes from saline and cisplatin (2 and 4 mg/kg) treated mice. Small oocytes were isolated from saline control mice, or 3, 6 and 24 h after cisplatin treatment and mitochondrial membrane potential was assessed using TMRM. A Representative confocal images of small oocytes from controls and 2 mg/kg cisplatin treated mice at 3, 6 and 24 h. Scale bar = 20 μm. B Relative TMRM/MitoTracker ratio in small oocytes with TMRM signal at 3 (n = 46/48), 6 (n = 35/29) and 24 (n = 41/44) hours after 2 mg/kg cisplatin. C Representative confocal images of small oocytes from saline treated controls and mice at 3, 6 and 24 h after 4 mg/kg cisplatin treatment. Scale bar = 20 μm. D Relative TMRM/MitoTracker ratio in small oocytes with TMRM signal at 3 (n = 45/50), 6 (n = 72/75) and 24 (n = 35/22) hours after 4 mg/kg cisplatin. E Percentage of small oocytes with low or without TMRM in control (n = 229) and 3 (n = 76), 6 (n = 80) and 24 (n = 68) hours after 4 mg/kg cisplatin treatment. F Magnification of small oocytes (indicated by the yellow arrow in C) without TMRM signal (a) and abnormal mitochondrial distribution (b). Scale bar = 10 μm. * p-value < 0.05, **p-value < 0.01, ****p-value < 0.0001

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