Fig. 5

Mitochondria in mature oocytes (4 mg/kg cisplatin injected at PN50). Mice were treated with saline (controls) or 4 mg/kg cisplatin at PN50 and then super ovulated 3 weeks later. a Number of oocytes harvested from mice treated at PN50. Each dot represents one animal, N = 6/12 for control and cisplatin groups, respectively. b Representative confocal images of mature oocytes stained with Mitotracker and TMRM. Scale bar = 50 μm. c Relative MitoTracker intensity of mature oocytes from untreated control mice (n = 29 oocytes) and 4 mg/kg cisplatin treated mice (n = 59 oocytes). d TMRM to MitoTracker ratio in mature oocytes from untreated control mice (n = 29 oocytes) and 4 mg/kg cisplatin treated mice (n = 59 oocytes). e ATP content in mature oocytes. N = 3/3 from control and cisplatin treated groups, respectively