Fig. 4

LINC00922 binds to miR-361-3p positively regulating the expression of CLDN1. a, b Cellular localization of LINC00922 in ovarian cancer cells was identified by RNA FISH and of cytoplasmic and nuclear RNA fractions assays. c The expression of miR-361-3p and CLDN1 was detected by RT-qPCR. d RT-qPCR assay demonstrated the expression of miR-361-3p in ovarian cancer cells through transfection with sh-linc00922. e The expression of CLDN1 were identified by performing RT-qPCR in that of ovarian cancer cells through transfection with either miR-361-3p mimics or miR-361-3p inhibitor. f Schematic illustration of LINC00922-WT and LINC00922-MUT luciferase reporter vectors, and the relative luciferase activities were detected in OC cells after transfection with LINC00922-WT or LINC00922-Mut and miR-361-3p mimics or NC mimics, respectively. g Schematic illustration of CLDN1-WT and CLDN1-MUT luciferase reporter vectors, and the relative luciferase activities were detected in OC cells after transfection with CLDN1-WT or CLDN1-MUT and miR-361-3p mimics or NC mimics, respectively. h, j The mRNA and protein expression of CLDN1 were identified in ovarian cancer cells after that of co-transfection with sh-linc00922 along with either miR-361-3p inhibitor or NC inhibitor. sh-linc00922, LINC00922 small hairpin RNA. sh-NC, corresponding negative control of sh-linc00922. miR inh., miR-361-3p inhibitor. NC inh., corresponding negative control of miR-361-3p inhibitor. *P < 0.05, **P < 0.01, ***P < 0.001 vs. sh-linc00922 + NC inh. ^###P < 0.001 vs. sh-linc00922 + NC inh. ^&&P < 0.01, ^&&&P < 0.001 vs. sh-NC + miR inhibitor