Fig. 3

The transcriptional activity of the V136L variant located in the propeptide region of BMP-15 was investigated in the granulosa derived COV434 cells transiently transfected with empty vector ± 100 ng recombinant human BMP-15 (pCS2 ± BMP-15) or wild type human BMP-15 expressing vector (WT) or the V136L variant vectors obtained by directed-mutagenesis of residues under positive selection. Results are expressed as the mean (± SD) of three independent experiments. Differences between means were analyzed with Student’s t-test comparing each condition to WT (*, p-value < 0.05)