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Table 1 Mean number of follicles after in vitro organotypic culture of fresh or cryopreserved whole ovaries

From: Pharmacological inhibition of the PI3K/PTEN/Akt and mTOR signalling pathways limits follicle activation induced by ovarian cryopreservation and in vitro culture

  Fresh SF
w/o cult Cult 2 days Cult 4 days w/o cult Cult 2 days Cult 4 days
Primordial 28,94 ± 2,614 22,99 ± 1,853 14,62 ± 1,958a,c 22,4 ± 1,175 14,78 ± 1,587b,c 11,13 ± 1,29b
Primary 17,68 ± 2,068 15,26 ± 1,541 16,95 ± 2,471 18,04 ± 1,026 11,16 ± 1,219b 12,48 ± 1,155b
Secondary or more 53,37 ± 3,503 61,75 ± 2,48 68,44 ± 3,757a 59,56 ± 1,798 74,05 ± 2,346b,c 76,39 ± 2,163b
  1. Fresh or SF (slow frozen/thawed) ovaries from FVB/N mice, 7-weeks old, n = 6–9 ovaries per group. w/o cult = without culture
  2. Results are expressed in percentage of primordial, primary and secondary or more mature follicles relative to the total number of follicles present in one ovarian slide. Mean ± SEM
  3. adifferent from Fresh w/o cult, bdifferent from SF w/o cult, cdifferent from Fresh cult 2 days