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Table 1 Mean number of follicles after in vitro organotypic culture of fresh or cryopreserved whole ovaries

From: Pharmacological inhibition of the PI3K/PTEN/Akt and mTOR signalling pathways limits follicle activation induced by ovarian cryopreservation and in vitro culture

 

Fresh

SF

w/o cult

Cult 2 days

Cult 4 days

w/o cult

Cult 2 days

Cult 4 days

Primordial

28,94 ± 2,614

22,99 ± 1,853

14,62 ± 1,958a,c

22,4 ± 1,175

14,78 ± 1,587b,c

11,13 ± 1,29b

Primary

17,68 ± 2,068

15,26 ± 1,541

16,95 ± 2,471

18,04 ± 1,026

11,16 ± 1,219b

12,48 ± 1,155b

Secondary or more

53,37 ± 3,503

61,75 ± 2,48

68,44 ± 3,757a

59,56 ± 1,798

74,05 ± 2,346b,c

76,39 ± 2,163b

  1. Fresh or SF (slow frozen/thawed) ovaries from FVB/N mice, 7-weeks old, n = 6–9 ovaries per group. w/o cult = without culture
  2. Results are expressed in percentage of primordial, primary and secondary or more mature follicles relative to the total number of follicles present in one ovarian slide. Mean ± SEM
  3. adifferent from Fresh w/o cult, bdifferent from SF w/o cult, cdifferent from Fresh cult 2 days
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Journal of Ovarian Research

ISSN: 1757-2215

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