|
Fresh
|
SF
|
---|
w/o cult
|
Cult 2 days
|
Cult 4 days
|
w/o cult
|
Cult 2 days
|
Cult 4 days
|
---|
Primordial
|
28,94 ± 2,614
|
22,99 ± 1,853
|
14,62 ± 1,958a,c
|
22,4 ± 1,175
|
14,78 ± 1,587b,c
|
11,13 ± 1,29b
|
Primary
|
17,68 ± 2,068
|
15,26 ± 1,541
|
16,95 ± 2,471
|
18,04 ± 1,026
|
11,16 ± 1,219b
|
12,48 ± 1,155b
|
Secondary or more
|
53,37 ± 3,503
|
61,75 ± 2,48
|
68,44 ± 3,757a
|
59,56 ± 1,798
|
74,05 ± 2,346b,c
|
76,39 ± 2,163b
|
- Fresh or SF (slow frozen/thawed) ovaries from FVB/N mice, 7-weeks old, n = 6–9 ovaries per group. w/o cult = without culture
- Results are expressed in percentage of primordial, primary and secondary or more mature follicles relative to the total number of follicles present in one ovarian slide. Mean ± SEM
- adifferent from Fresh w/o cult, bdifferent from SF w/o cult, cdifferent from Fresh cult 2 days