Fig. 5

FZD4 was targeted by miR-331-3p and positively regulated by hsa_circ_0004712. A The interaction between miR-331-3p and FZD4 was predicted by starBase. B and C The interaction between miR-331-3p and FZD4 was validated by dual-luciferase reporter assay and RIP assay. D The expression of FZD4 in cells transfected with miR-331-3p, NC, miR-331-3p + hsa_circ_0004712 or miR-331-3p + circ-NC was detected by western blot. E and F FZD4 expression in OC tissues was detected by qRT-PCR and western blot. G FZD4 expression in OC tissues was determined by IHC staining analysis. H The expression of FZD4 in OC cells was detected by western blot. I and J The association between FZD4 expression and hsa_circ_0004712 expression or miR-331-3p expression was analyzed by Spearman’s test. K The association between FZD4 and miR-331-3p expression was obtained from starBase. *P < 0.05