Fig. 6

MiR-331-3p played anti-tumor role in OC by suppressing FZD4. OVCAR-3 and SKOV-3 cells were transfected with miR-331-3p, NC, miR-331-3p + FZD4 or miR-331-3p + vector. A The expression of FZD4 detected by western blot. B Cell proliferation assessed by CCK-8 assay. C Colony formation ability assessed by colony formation assay. D Cell apoptosis assessed by flow cytometry assay. E Cell invasion assessed using transwell assay. F Cell migration assessed using wound healing assay. G and H The expression of C-caspase 3, MMP2 and MMP9 measured by western blot. I and J The activities of MMP2 and MMP9 were determined by gelatin zymography analysis. *P < 0.05