Table 1 Transport of human ovaries for cryopreservation in two types of media. Volume of ovaries and pH of media. Number of oocytes found in connection with ovarian tissue cryopreservation and in vitro maturation results
From: Histidine buffered media maintains pH stabile during cooled transportation of human ovarian tissue
Transport media | p-values | ||
|---|---|---|---|
Histidine buffered | HEPES buffered | ||
Patients (n) | 16 | 18 | |
Age/years (mean ± SD) | 27.3 ± 5.9 | 29.0 ± 5.9 | 0.4 |
Ovarian volume/ml (mean ± SD) | 7.0 ± 2.7 | 6.9 ± 2.8 | 0.6 |
pH (mean ± SD) | 7.2 ± 0.05 | 7.5 ± 0.13 | Mean: < 0.001 SD: p < 0.0001 |
Results from in vitro maturation | |||
Patients (n) | 7 | 8 | |
Age/years (mean ± SD) | 25.8 ± 5.9 | 29.4 ± 6.1 | 0.3 |
Ovarian volume/ml (mean ± SD) | 7.4 ± 2.7 | 6.8 ± 3.2 | 0.7 |
pH (mean ± SD) | 7.2 ± 0.05 | 7.5 ± 0.10 | Mean: < 0.001 SD: p < 0.001 |
Total oocytes (n) | 247 | 170 | |
MII after 48 h (n) | 67 | 43 | 0.7 |