Fig. 4
From: Targeting novel coronavirus SARS-CoV-2 spike protein with phytoconstituents of Momordica charantia
Binding kinetics analysis of SARS-CoV-2 spike protein and the bitter melon phytoconstituent, erythrodiol. Surface plasmon resonance (SPR) sensogram showing the binding kinetics for immobilized SARS-CoV-2 spike protein S2 and the bitter melon phytoconstituent, erythrodiol. A typical response curve at different analyte concentrations (green, 1 μM; black, 10 μM; red, 20 μM; blue, 50 μM) shows the association (ka = 1.93 × 103 M-1S-1) and dissociation (kd = 2.21 × 10 − 3 S-1) rate constant of interaction. A binding affinity between SARS-CoV-2 spike protein S2 and erythrodiol showed an equilibrium dissociation constant (KD) value, 1.15 μM. Data sets were analyzed by one-to-one (1:1) binding fit models using TraceDrawer evaluation software