Fig. 2

Overexpression of GSTT1 in drug-resistance SOC. A. The expression level of GSTT1 and other taxol and carboplatin resistance-related molecules was assessed by qRT-PCR(a) and Western blot(b). GSTT1, Topo I, and Cyclin E1 were up-regulated in drug-resistant HO8910 and SKOV3 cells. B. Oncomine data analysis for GSTT1 in SOC. (a) GSTT1 was over-expressed in SOC, compared to the most of other cancer samples. (b) GSTT1 was over-expressed in SOC, compared to normal tissues. C. The transcriptional level of GSTT1 in OC tissues (n = 40) and normal tissues (n = 20) were determined by qRT-PCR and normalized by the internal control β-actin gene. The bars in the figure indicated the median of the relative expression of GSTT1. D. Immunostaining of GSTT1 protein with GSTT1 antibody in SOC tissues (a-b): Case 1, TAX-sensitive SOC showed weak staining of GSTT1, while TAX-resistant SOC showed much more positive staining of GSTT1. (c-d): Case 2, TAX-sensitive SOC(c) and -resistance SOC (d) showed images of weak and strong GSTT1 expression, respectively