Fig. 3

Down-regulation of GSTT1 increased the sensitivity of drug-resistant cells to taxol and carboplatin. Knockdown efficiency of GSTT1 was determined by qRT-PCR analysis (A) and Western-blot (B) in HO8910 cells resistant to taxol or (and) carboplatin. C The proliferation of HO8910 stable cell lines was tested by CCK-8 assay in the absence of taxol and carboplatin. D The colony formation was assessed using HO8910 stable cell lines in the absence of taxol and carboplatin. E Inhibition of GSTT1 increased the sensitivity of taxol and carboplatin resistant cell lines to the treatment of 50 μg/ml taxol or 100 μg/ml carboplatin, or to combined treatment of these two drugs for 48 h, in CCK-8 assays and colony formation assays (F)