Table 1 Sequences of primers and targets
From: Up-regulation of GSTT1 in serous ovarian cancer associated with resistance to TAXOL / carboplatin
Primers/targets | Sequences |
|---|---|
GSTT1 qRT-PCR forward | 5′-CCAAGCTGCACGATAGGTCAC-3′ |
GSTT1 qRT-PCR reverse | 5′-GGTATGCTACACACAGCTCCAC-3′ |
MDR1 qRT-PCR forward | 5′-GACATGACCAGGTATGCCTA-3′ |
MDR1 qRT-PCR reverse | 5′-CTTGGAGACATCATCTGTAAGTC-3′ |
Topo I qRT-PCR forward | 5′-AGGTTCCTTCTCCTCCTCCA-3′ |
Topo I qRT-PCR reverse | 5′-GCCGAGCAGTCTCGTATTTC-3′ |
ERCC1 qRT-PCR forward | 5′-CCTTATTCCGATCTACACAGAGC-3′ |
ERCC1 qRT-PCR reverse | 5′-TATTCGGCGTAGGTCTGAGGG-3’ |
Bcl2 qRT-PCR forward | 5′-TTGCCAGCCGGAACCTATG-3’ |
Bcl2 qRT-PCR reverse | 5′-CGAAGGCGACCAGCAATGATA-3’ |
Cyclin B1 qRT-PCR forward | 5′-AATAAGGCGAAGATCAACATGGC-3’ |
Cyclin B1 qRT-PCR reverse | 5′-TTTGTTACCAATGTCCCCAAGAG-3’ |
Cyclin E1 qRT-PCR forward | 5′-GCCAGCCTTGGGACAATAATG-3’ |
Cyclin E1 qRT-PCR reverse | 5′-CTTGCACGTTGAGTTTGGGT-3’ |
β-actin qRT-PCR forward | 5′- AAGGTGACAGCAGTCGGTT-3’ |
β-actin qRT-PCR reverse | 5′- TGTGTGGACTTGGGAGAGG-3’ |
shGSTT1–1 forward | 5′-CCGGCAGCACTTAAGCGATGCCTTTCTCGAGAAAGGCATCGCTTAAGTGCTGTTTTTG-3’ |
shGSTT1–1 reverse | 5′-AATTCAAAAACAGCACTTAAGCGATGCCTTTCTCGAGAAAGGCATCGCTTAAGTGCTG-3’ |
shGSTT1–2 forward | 5′-CCGGGCTTGCTTAAGACTTGCCCAACTCGAGTTGGGCAAGTCTTAAGCAAGCTTTTTG-3’ |
shGSTT1–2 reverse | 5′-AATTCAAAAAGCTTGCTTAAGACTTGCCCAACTCGAGTTGGGCAAGTCTTAAGCAAGC-3’ |