Fig. 5

MAP3K3 expression was modulated by circSETDB1 through acting as a target gene of miR-129-3p in A2780 and SKOV3 cells. A The binding sequence between miR-129-3p and MAP3K3 as well as the mutated sites of MAP3K3 were shown. B The binding relationship between miR-129-3p and MAP3K3 was identified by dual-luciferase reporter assay in 293 T cells. C MAP3K3 mRNA expression was detected by qRT-PCR in 73 pairs of SOC tissues and healthy fallopian tube tissues. D and E The protein abundance of MAP3K3 was determined by western blot analysis in SOC tissues (N = 73), adjacent normal ovarian tissues (N = 73) and IOSE-80, A2780 and SKOV3 cells. F and G The correlation between MAP3K3 and miR-129-3p or circSETFB1 expression was disclosed by Spearman correlation analysis in SOC tissues. H The effects between circSETDB1 silencing and miR-129-3p inhibitor on MAP3K3 protein level were demonstrated by western blot analysis in A2780 and SKOV3 cells. *P < 0.05