Fig. 6

MiR-129-3p regulated SOC progression by interacting with MAP3K3. A MiR-129-3p expression was detected by qRT-PCR in both A2780 and SKOV3 cells transfected with miR-129-3p mimic or mimic NC. B Western blot analysis was performed to determine MAP3K3 protein abundance in both A2780 and SKOV3 cells transfected with pcDNA or pcDNA-MAP3K3. C-I Both A2780 and SKOV3 cells were severally transfected with mimic NC, miR-129-3p mimic, miR-129-3p mimic + pcDNA and miR-129-3p mimic + pcDNA-MAP3K3, respectively. C MAP3K3 protein abundance was detected by western blot analysis. D and E CCK-8 and EDU assays were conducted to investigate cell proliferation. F Cell apoptosis was determined by flow cytometry analysis. G and H Cell invasion and migration were revealed by transwell invasion and wound-healing assays, respectively. I MMP9 and MMP2 protein abundance were detected by western blot analysis. *P < 0.05