Fig. 2

UBE2S promotes the proliferation of ovarian cancer cells. A2780 and SKOV3 cells were stably transfected with PLKO.1 (Ctr), UBE2S shRNA1 (sh-1), UBE2S shRNA2 (sh-2), PCMV (Ctr), and PCMV-UBE2S (UBE2S). A Western blot was performed to detect the protein level of UBE2S and β-actin in A2780 and SKOV3. B Quantification of relative protein levels of UBE2S in (A). The CCK8 assay was conducted to analyze the proliferation of A2780 (C, D) and SKOV3 (E, F) cells with UBE2S overexpression or knockdown. G Clonogenic assay was used to determine the colony formation efficiency of A2780 and SKOV3 cells. H Quantification of the number of clones in (G). A2780 and SKOV3 cells were stably transfected with PCMV, PCMV-UBE2S (UBE2S), and PCMV-UBE2S-C95S (C95S). I and J The CCK8 assay was conducted to analyze cell proliferation. K Clonogenic assay was used to determine the colony formation efficiency of A2780 and SKOV3 cells. Quantification of the number of clones was shown in Additional file 2. (Data are mean ± SEM, *p < 0.05, **p < 0.01, n = 3)