Fig. 6

UBE2S induces Wnt/β-catenin activation in ovarian cancer cells. A Western blot was conducted to detect the protein levels of β-catenin, c-Myc, cyclin D1, and β-actin in A2780 and SKOV3 cells transfected with PLKO.1 (Ctr), UBE2S shRNA1 (sh-1), UBE2S shRNA2 (sh-2), PCMV (Ctr), and PCMV-UBE2S (UBE2S). B and C Quantification of relative protein levels of β-catenin, c-Myc, and cyclin D1 in (A). D Immunofluorescence staining was performed to detect the nuclear translocation of β-catenin (Red) in A2780 cells with UBE2S overexpression. Nuclei were counterstained with DAPI (Blue). Scale bar: 10 μm. E Quantification of the percentage of β-catenin nuclear translocation in (D). F A2780 and SKOV3 cells transfected with PCMV and PCMV-UBE2S (UBE2S) were treated with or without TAME (10 μM) for 24 h. Western blot was conducted to detect the protein levels of β-catenin, c-Myc, cyclin D1, and β-actin. G Quantification of the relative protein levels in (F). (Data are mean ± SEM, *p < 0.05, **p < 0.01, n = 3)