Fig. 23

(a-b) Mouse bone marrow VSELs and HSCs also express FSHR. (c) Also note co-expression of a stem cell marker SCA-1 and FSHR. (d-e) Mouse decalcified bone sections after chemoablation (5-flurouracil, 150 mg/kg) to study the effect of FSH on resumption of hematopoiesis. Left panel in d & e are FSH treatment to chemoablated mice on D4 and D7 compared to no treatment in the right panel. FSH enhanced hematopoiesis by almost 72 h by exerting a direct action on VSELs [72]. (f) As evident in 5-FU treated mouse bone marrow, 10-fold increase in FSHR3 was observed 6 h after FSH (5 IU) treatment [56]. (g) Ovariectomized and castrated mice were treated with Estradiol (E2, 2 μg/day), progesterone (P, 1 mg/Kg/day) and FSH (5 IU per day) for 7 days and absolute numbers of VSELs were enumerated. FSH exerted a direct action and VSELs numbers were increased similar to after E2 treatment whereas P did not exert any effect [77]. Scale: 20 μm