Fig. 3

Knockdown of TTK expression in cisplatin-resistant cells would suppress cell proliferation and increase the drug sensitivity to cisplatin. A The knockdown of TTK in A2780cis cell line was verified by western blot and quantitative real-time PCR. B The cell proliferation of A2780cis cells with or without TTK knockdown was assayed by CCK8 kit. The growth of A2780cis cells with TTK knockdown was suppressed, especially in combination with cisplatin treatment. C DNA synthesis detection by EdU staining. The red fluorescence represents the ability of cell proliferation. D Colony formation assay showed that TTK inhibitor performed the same function as TTK knockdown. The clones were reduced when TTK was inhibited or up-regulated, especially in combination with cisplatin treatment. E Down-regulation of TTK or TTK inhibitor could significantly increase cell apoptosis rates with cisplatin treatment. *P < 0.05, **P < 0.01, ***P < 0.001