Fig. 4

Confirmation of PARP1i-induced protein networks in HGSOC cell lines. Protein network and pathway analysis were performed on independent L1000 transcriptome dataset for HGSOC cell lines RMUGS, COV644 and TYKNU treated with pan-PARPi olaparib and rucaparib as described previously (FDR-corrected p-value < 0.05; Material and Methods, STable3). (a, b) Barplots represents top 5 frequently responding annotated protein modules (adj. p-value < 0.05) across all cell lines (STable3). (c) Correlation analysis of the overall protein module activity based on overlapping annotated modules between L1000 olaparib-treated RMUGS cell line and proteome profiling. Activity was calculated by integrating protein expression changes in modules [38] (Methods)