Fig. 5

MiR-136 bound to CBX2 in PTX-resistant SKOV3 and HeyA8 cells. (A) Starbase online database was employed to predict the binding sites between miR-136 and CBX2. (B and C) Luciferase activity was detected by dual-luciferase reporter assay in PTX-resistant SKOV3 and HeyA8 cells. (D-G) The mRNA and protein expressions of CBX2 were detected by qRT-PCR and western blot in PTX-resistant ovarian cancer tissues (N = 20), PTX-sensitive ovarian cancer tissues (N = 19), SKOV3 and HeyA8 cells as well as PTX-resistant SKOV3 and HeyA8 cells. (H and I) The effects of circ_0061140 knockdown on the mRNA and protein expressions of CBX2 were demonstrated by qRT-PCR and western blot, respectively, in PTX-resistant SKOV3 and HeyA8 cells. (J) Spearman correlation analysis was conducted to elucidate the linear relationship between CBX2 and circ_0061140 expressions. (K-M) The influences between miR-136 mimic and CBX2 on the mRNA and protein expressions of CBX2 were demonstrated by qRT-PCR and western blot, respectively, in PTX-resistant SKOV3 and HeyA8 cells. *P < 0.05