Fig. 3

SNHG20 up-regulated ROCK1 level by sponging miR-148a. (A) The expression of ROCK1 and miR-148a was measured by qPCR; (B) The protein level of ROCK1 was detected by Western blotting; (C) Predicted binding site between miR-148a and SNHG20; (D) Predicted binding site between miR-148a and ROCK1; (E) The direct binding between miR-148a and SNHG20 was validated by dual luciferase assay; (F) The direct binding between miR-148a and ROCK1 was validated by dual luciferase assay. (G) The protein expression of ROCK1 in the normal ovarian epithelial cells and OC cells was measured by Western blotting. (H) Significant higher level of ROCK1 was found in OC cells. *P < 0.05, **P < 0.01, ***P < 0.001