Fig. 4

Knockdown of SNHG20 suppressed the proliferation and promoted apoptosis of OC cells via up-regulating miR-148a. (A) The expression of miR-148a was measured by qPCR after transfection with miR-148a mimics or miR-148a inhibitor; (B) The mRNA expression of ROCK1 was measured by qPCR after transfection with miR-148a mimics or miR-148a inhibitor; (C) The protein expression of ROCK1 was detected by western blotting after transfection with miR-148a inhibitor or miR-148a mimics; (D) Quantitative analysis of the protein bands; (E) Cell proliferation was assessed by colony formation assay; (F) Quantification analysis of cell proliferation after transfection with sh-SNHG20 and miR-148a inhibitor; (G) Cell apoptosis was detected by flow cytometry; (H) Quantification analysis of cell apoptosis after transfection with sh-SNHG20 and miR-148a inhibitor. *P < 0.05, **P < 0.01, ***P < 0.001