Fig. 3
From: The challenge of ovarian tissue culture: 2D versus 3D culture
Follicular analysis. Representative images of ovarian tissue (C scale bar: 500 μm) and morphological normal primordial (G), primary (H) and secondary (I) follicles (arrow, scale bar: 50 μm). Density of follicles classified as normal (A) and degenerated (B) according to oocyte and granulosa cell morphology. Density of primordial (D), primary (E) and secondary (F) follicles. A linear regression was performed for 2D and 3D groups and their corresponding slopes show an increase over time in atretic follicles and a decrease in primordial follicles. Additionally, a comparison between 2D and 3D at 48 and 72 h was made using the t-test, which shows statistical differences for the density of primary follicles at 72 h. Original magnification: A × 100; B × 400; H and I × 200. Data presented as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001