Fig. 5
From: The challenge of ovarian tissue culture: 2D versus 3D culture
Tissue proliferation evaluated through Ki67 staining in follicles (A) and stromal cells (B). Representative images of Ki67 staining in a secondary follicle (C) and stromal cells (E). A negative primary follicle (arrow) is represented in D. A linear regression was performed for 2D and 3D groups and their corresponding slopes show a decrease over time in stromal cell proliferation for 3D group. Original magnification × 200 (C, E) and × 400 (D), scale bar 50 μm. Data presented as mean ± SEM. ***p < 0.001