Fig. 1

miR-425-5p overexpression in ovarian cancer is negatively regulated by MAGI2-AS3. A Venn diagram showing the overlapping of the predicted target miRNAs and upregulated miRNAs in GSE83693. GSE83693 contained four normal ovarian tissue samples and 16 ovarian cancer samples. B GSE119055 datasets were used to verify the expression levels of four miRNAs in ovarian cancer tissues and normal ovarian tissues. GSE119055 contained three normal ovarian tissue samples and six ovarian cancer samples. C Correlation of MAGI2-AS3 and miR-425-5p expression. DmiR-425-5p expression was detected by real-time quantitative polymerase chain reaction (RT-qPCR) in ovarian cancer cells (A2780, SKOV3, and ES-2) and SV-40 transformed human ovarian epithelial cells (IOSE-80) cells. EMiR-425-5p expression levels were determined by RT-qPCR in SKOV-3 and ES-2 cells, after transient transfection of MAGI2-AS3 and its negative control (NC). F Schematic diagram showing the binding sites of miR-425-5p and MAGI2-AS3 3’UTR and the mutation site MAGI2-AS3 3’UTR. G Relative luciferase activities were detected by dual-luciferase reporter assays in SKOV-3 and ES-2 cells after co-transfection of miR-425-5p mimics or NC mimics with wild-type-MAGI2-AS3-luc (WT) and mutant-MAGI2-AS3-luc (MUT). ^N.S.p > 0.05, ^#p < 0.05, ^##p < 0.01, ^###p < 0.001