Fig. 5

Silencing AAF4 against miR-425-5p affects the proliferation, invasion, and migration of ovarian cancer cells. A The Cell Counting Kit-8 assay was used to detect cell proliferation in transfected SKOV-3 and ES-2 cells. B, C The wound-healing assay was used to detect cell migrations in transfected SKOV-3 and ES-2 cells. D, E The Transwell assay was used to detect cell invasions in transfected SKOV-3 and ES-2 cells. Bar = 100 μm. sh-AFF4, AFF4 small hairpin RNA; sh-NC, negative control of sh-AFF4; miR inhibitor, miR-425-5p inhibitor; NC inhibitor, negative control of miR-425-5p inhibitor. ^#p < 0.05, ^###p < 0.001, ^$p < 0.05, and ^$$p < 0.01 comparison with miR inhibitor + sh-NC. ^&p < 0.05, ^&&p < 0.01, and ^&&&p < 0.001 comparison with NC inhibitor + sh-AFF4