Fig. 1

Comparison of ID8-L4 and ID8-L11 cells for linearity of bioluminescence output and cisplatin sensitivity in vitro. A Graphical representation of concentration-dependent bioluminescence imaging. Different concentrations of ID8-L11 and ID8-L4 cells were seeded into a 96-well plate and imaged on an IVIS spectrum in vivo imaging system. Measured values are shown with solid lines. Linear fitting of the data was performed and is represented by dashed lines. B-D ID8 (B), ID8-L11 (C) and ID8-L4 (D) cells were seeded in 96 well plates and 24 h later were treated with different concentrations of cisplatin (0, 10, 25 or 50 μM). Cell viability was determined by XTT dye reduction assays at the time points indicated. Data shown indicate the mean ± SEM of 4 independent replicates. Within each time point, groups with different letters are statistically different from one another as determined by ANOVA followed by Fisher’s LSD test (p<0.05)