Fig. 3

Expression of genes related to steroidogenesis. GCs were cultured for 7 days to form a monolayer. On day 8, a monolayer of GCs was cocultured with COCs either with or without the supplementation with the high level of NEFAs during in vitro maturation. The transcript abundance of different genes related to estradiol and progesterone was determined after IVM. The expressions of STAR, HSD3B1, and CYP19A3 was not affected by the high level of NEFAs. NC is the control group, and FA is the group exposed to the elevated level of NEFA. STAR: Steroidogenic Acute Regulatory Protein; CYP11A1: Cytochrome P450 Family 11 Subfamily A Member 1; HSD3B1: Hydroxy-Delta-5-Steroid Dehydrogenase, 3 Beta- And Steroid Delta-Isomerase 1; CYP19A3: Cytochrome P450 Family 19 Subfamily A 3