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Table 1 Information on RT-qPCR primers

From: Cocultured porcine granulosa cells respond to excess non-esterified fatty acids during in vitro maturation

Target gene

Accession number

Forward primer (5’-3’)

Reverse primer (5’-3’)

Product size

Annealing Temperature

AGPS

XM_021075128.1

GACGCAGACTTATGATGCAGG

AGCCACTGCTTCCGTAACT

180 bp

57 °C

MAP3K8

XM_021064737.1

ATGGAACCGTGGAGGACTTG

CGGAGCCGATATTCCTGTAAGT

192 bp

57 °C

TLR6

NM_213760.2

CTCTCATGGCACAGCGAACT

CACATCATCCTCTTCAGCGACT

125 bp

59 °C

AGO1

XM_005665209.3

ACGCTGTTACCTCACTGGATAG

GGCCAGATGTGACAAGATGAGG

244 bp

59 °C

IL33

NM_001285978.1

ATGGTGGTGGTGATCATCGGA

ACACTCCAGGATGGCTCTTACA

201 bp

59 °C

GAPDH

NM_001206359.1

ACTGGTGTCTTCACGACCAT

GGAGGCATTGCTGACGATCT

159 bp

57 °C

STAR

NM_213755.2

CCCTTGGACAGGAGCTGAAC

GGCCAGATCTTGGTCACTGT

134 bp

57 °C

CYP11A1

NM_214227.1

AGACACTGAGACTCCACCCCA

GACGGCCACTTGTACCAATGT

110 bp

57 °C

HSD3B1

NM_001004049.2

GATCGTCCACTTGTTGCTGG

GATCTTGCTCTGGAGCTTAGAA

109 bp

57 °C

CYP19A3

NM_214431.1

GTGGACGTGTTGACCCTCAT

TATGCACGAGGGCACTTTCG

94 bp

57 °C

SDHA [61]

XM_021076930.1

CACACGCTTTCCTATGTCGATG

TGGCACAGTCAGCTTCATTC

94 bp

57 °C

PPIA [61]

NM_214353.1

AAAACTTCCGTGCTCTGAGC

TTATGGCGTGTGAAGTCACC

112 bp

59 °C

  1. Note: The superscript number beside a target gene indicates the reference from which the primer sequence was obtained