Skip to main content
Fig. 5 | Journal of Ovarian Research

Fig. 5

From: Long noncoding RNA SNHG25 promotes the malignancy of endometrial cancer by sponging microRNA-497-5p and increasing FASN expression

Fig. 5

si-SNHG25 lowered FASN level by trapping miR-497-5p. A The interacting sequences between miR-497-5p and FASN 3′-UTR. B Luciferase activity was tested in EC cells transfected with FASN-wt or FASN-mut in parallel with miR-497-5p/NC mimic. C, D FASN levels were quantified in EC cells after miR-497-5p upregulation. E The expression status of FASN in EC tissues. F The expression relationship between FASN and miR-497-5p in EC tissues. G The expression relationship between FASN and SNHG25 in EC tissues. H, I FASN levels were measured in SNHG25-deficient EC cells. J, K si-SNHG25, together with anti-miR-497-5p or anti-NC, was transfected into EC cells, followed by the determination of FASN expression. L RIP assay was conducted to confirm the enrichment of SNHG25, miR-497-5p, and FASN in immunoprecipitated RNA. **P < 0.01

Back to article page