Fig. 4

MNX1-AS1 binds to miR-744-5p and inhibits its expression. A RT-qPCR detected MNX1-AS1 expression in the cytoplasm and nucleus of OC cells. B The predicted binding sequence of miR-744-3p and MNX1-AS1, and the mutated binding sequence of MNX1-AS1. C Luciferase activity was detected in SKOV-3 and OVCAR-3 cells co-transfected with MNX1-AS1 WT or MNX1-AS1 Mut vector and NC mimics or miR-744-5p mimics. D RT-qPCR detected the expression of miR-744-5p in OC non-tumor and tumor tissues. E RT-qPCR detected the expression of miR-744-5p in OC cells and normal ovarian cells. F RT-qPCR detected the expression of miR-744-5p in SKOV-3 and OVCAR-3 cells transfected with sh-MNX1-AS1 or sh-NC. G Correlation analysis of MNX1-AS1 and miR-744-5p expression levels in OC tumor tissues. ^**P<0.01,^***P<0.001, the difference comparison was compared with control group cells and tissues. Error bars were represented the mean ± SD of triplicate experiments