Fig. 7

The function of MNX1-AS1/ miR-744-5p/ SOX12 axis in OC. MNX1-AS1 shRNA lentivirus was transfected into SKOV-3 and OVCAR-3 cells, respectively, to construct MNX1-AS1 knockdown stable strain. NC inhibitor, miR-744-5p inhibitor, NC shRNA and SOX12 shRNA were transfected into SKOV-3/sh-MNX1-AS1 and OVCAR-3/sh-MNX1-AS1 cells, respectively. The knockdown efficiency of miR-744-5p and SOX12 was detected by RT-qPCR (A). MiR-744-5p inhibitor and SOX12 shRNA were transfected into SKOV-3/sh-MNX1-AS1 and OVCAR-3/sh-MNX1-AS1 cells simultaneously. B Cell viability was detected by CCK8 assay. C Colony formation assay was used to detect cell proliferation. D Cell apoptosis was detected by flow cytometry. E Transwell assay was used to detect cell migration and invasion. ^*P<0.05, ^**P<0.01, the difference comparison was compared with NC inhibitor or NC shRNA transfected cells. ^#P<0.05, the difference comparison was compared with miR-744-5p inhibitor transfected cells. Error bars were represented the mean ± SD in three independent repetitions