Mitochondria membrane damage was observed in the GCs of the PCOS patients. A Mitochondrial ultrastructure detected by TEM (n = 3 in each group). Mitochondrial swelling and mitochondrial membrane defect were observed in the PCOS group. The arrows point at mitochondria. B-D The p-Akt protein level was decreased in the PCOS patients while total Akt expression has no difference (n = 6 in each group). *P < 0.05, **P < 0.01, ***P < 0.001.