Fig. 5

Wnt/β-catenin signaling is involved in CXCL14-facilitated ovarian OC cell EMT and invasion. A Western blot analysis of the indicated expression levels (β-catenin, cyclin D1, MMP-7, Axin2) in CXCL14 overexpressing SKOV3 and OVCAR3 cells treated with XAV939 (10 μM). B Determination of the proliferative capability of CXCL14 overexpressing SKOV3 and OVCAR3 cells treated with XAV939 using the CCK-8. C Determination of the proliferative capability of CXCL14 overexpressing SKOV3 and OVCAR3 cells treated with XAV939 using the colony formation assay. D Determination of the migration capability of OC cells using the wound healing assay. Scale bar: 200 μm. E Determination of the invasive capability of OC cells using the Transwell assay. Scale bar: 200 μm. ^**P < 0.01 compared with vector, ^##P < 0.01 compared with CXCL14-OE. F-H Statistical analysis of the correlation between CXCL14 and Wnt/β-catenin target genes (Cyclin D1, MMP-7, Axin2) in OC patients (n = 379) cohort using TCGA database