Fig. 6

SMG1 was a target of miR-320a. A and B SMG1 level was measured in tumor and normal tissues (n = 35). C and D SMG1 level was examined in SKOV3, A2780 and IOSE-80 cells. E The linear correlation of miR-320a and SMG1 in ovarian cancer tissues was analyzed. F and G SMG1 abundance was detected in SKOV3 and A2780 cells after exposure to various concentrations of curcumin. H The binding site of miR-320a and SMG1 was predicted via starBase. I Luciferase activity was detected in 293 T cells transfected with SMG1 3’UTR wt or mut and miR-320a mimic or mimic NC. J The interaction between miR-320a and SMG1 was analyzed by RIP assay. K-L SMG1 abundance was measured in SKOV3 and A2780 cells transfected with si-NC or si-SMG1. ^*P < 0.05