Fig. 3

Circ_0000745 directly binds to miR-3187-3p in OC cells. A, sub-cellular localization of circ_0000745 in OC cells examined by a nuclear-cytoplasmic RNA separation assay; B, intersections of the candidate target miRNAs of circ_0000745 predicted using StarBase and CircBank; C, correlations between the candidate miRNAs and the survival rate of OC patients predicted on the Pan-cancer platform on StarBase; D, miR-3187-3p expression in the collected tissue samples detected by RT-qPCR; E, a negative correlation between the expression of miR-3187-3p and circ_0000745 in OC tissues; F, expression of miR-3187-3p in ES-2 and SK-OV-3 cells after oe-circ or sh-circ transfection determined by RT-qPCR; G, transfection efficacy of miR-mimic in 293 T cells confirmed by RT-qPCR; H, putative binding site between miR-3187-3p and circ_0000745 and the MT sequence for luciferase assay; I, luciferase activity of circ-WT and circ-MT in 293 T cells examined by the luciferase reporter gene assay. Data were exhibited as mean ± SD from three repetitions. Differences were analyzed by paired t test (D), unpaired t test (G), one-way ANOVA (F) or two-way ANOVA (I); *p < 0.05 vs. adjacent; @ p < 0.05 vs. NC mimic; #p < 0.05 vs. oe-NC; &p < 0.05 vs. sh-NC; In panel E, the correlation between miR-3187-3p and circ_0000745 was validated using Pearson’s correlation analysis, r = − 0.653, p < 0.001