Skip to main content
Fig. 7 | Journal of Ovarian Research

Fig. 7

From: RNA-binding protein IGF2BP2 enhances circ_0000745 abundancy and promotes aggressiveness and stemness of ovarian cancer cells via the microRNA-3187-3p/ERBB4/PI3K/AKT axis

Fig. 7

IGF2BP2 binds to circ_0000745 and promotes its expression in OC cells. A, candidate RBPs with the potential to regulate circ_0000745 predicted using StarBase; B, expression of IGF2BP2 in OC patients predicted on GEPIA; C, IGF2BP2 expression in collected OC tissues examined by RT-qPCR; D, correlation between the expression of IGF2BP2 and circ_0000745 in OC cells examined by RT-qPCR; E, expression of IGF2BP2 and circ_0000745 in ES-2 and SK-OV-3 cells examined by RT-qPCR; F, protein level of IGF2BP2 in ES-2 and SK-OV-3 cells after oe-IGF2BP2 or sh-IGF2BP2 transfections determined by western blot analysis; G, direct binding relationship between circ_0000745 and IGF2BP2 examined by an RNA pull down assay. Data were exhibited as mean ± SD from three repetitions. Differences were analyzed by paired t test (C), one-way ANOVA (F), or two-way ANOVA (E); *p < 0.05 vs. adjacent; @@p < 0.01 vs. Bio-NC; #p < 0.05 vs. oe-NC; &p < 0.05 vs. sh-NC; In panel D, the correlation between circ_0000745 and IGF2BP2 was validated using Pearson’s correlation analysis, r = − 0.729, p < 0.001

Back to article page