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Table 1 Primer sequences for RT-qPCR

From: RNA-binding protein IGF2BP2 enhances circ_0000745 abundancy and promotes aggressiveness and stemness of ovarian cancer cells via the microRNA-3187-3p/ERBB4/PI3K/AKT axis

Gene

Primer sequence (5′-3′)

circ_0000745

F: ATGTTGAAAGTAGCCCGAGCAG

R: TGGGAGTGTTGGAAGAAGTTGG

SPECC1

F: ACCCCAGGAAATCAGTGTCCA

R: GTTCCCGAACTTGGGACTCAA

miR-3187-3p

F: CCTGGGCAGCGTGTGGCT

R: CTCAACTGGTGTCGTGGA

ERBB4

F: GGAGTATGTCCACGAGCACAAG

R: CGAGTCGTCTTTCTTCCAGGTAC

IGF2BP2

F: GTTGGTGCCATCATCGGAAAGG

R: TGGATGGTGACAGGCTTCTCTG

GAPDH

F: GGGAAACTGTGGCGTGAT

R: GAGTGGGTGTCGCTGTTGA

U6

F: CTCGCTTCGGCAGCACA

R: AACGCTTCACGAATTTGCGT

  1. Note: RT-qPCR Reverse transcription quantitative polymerase chain reaction, SPECC1 Sperm antigen with calponin homology and coiled-coil domains 1, miR microRNA, ERBB4 Erb-b2 receptor tyrosine kinase 4, IGF2BP2, insulin like growth factor 2 mRNA binding protein 2, GAPDH Glyceraldehyde-3-phosphate dehydrogenase, F Forward, R Reverse
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Journal of Ovarian Research

ISSN: 1757-2215

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