Fig. 4

SIK1 overexpression inhibited OC progression. HEY and ES-2 cells were transfected with pc-DNA or pc-DNA SIK1 overexpression vector (SIK1). A-B The mRNA and protein expression levels of SIK1 were examined using qRT-PCR and WB analysis. The EDU positive cells, the number of colonies, the numbers of migrated and invaded cells, and the apoptosis rate of cells were determined using EDU staining (C), colony formation assay (D), transwell assay (E-F) and flow cytometry (G). H WB analysis was used to detect the protein expression levels of E-cadherin, N-cadherin, MMP9 and Bax. **P < 0.01, ***P < 0.001