Fig. 7
From: Circ_0078607 inhibits the progression of ovarian cancer via regulating the miR-32-5p/SIK1 network
SIK1 was a target of miR-32-5p. A The expression of candidate genes was detected by qRT-PCR in HEY and ES-2 cells transfected with miR-NC or miR-32-5p mimic. B The binding sites between SIK1 3’UTR and miR-32-5p were exhibited. C Dual-luciferase reporter assay was used to confirm the interaction between SIK1 and miR-32-5p. D-E QRT-PCR and WB analysis were used to measure the mRNA and protein expression of SIK1 in HEY and ES-2 cells transfected with miR-NC or miR-32-5p. F The correlation between SIK1 and miR-32-5p in OC tumor tissues (n = 43) was assessed by Pearson correlation analysis. G-H HEY and ES-2 cells were transfected with NC, oe-circ_0078607, oe-circ_0078607 + miR-NC or oe-circ_0078607 + miR-32-5p. The mRNA and protein expression levels of SIK1 were examined using qRT-PCR and WB analysis. **P < 0.01, ***P < 0.001