Fig. 8
From: Circ_0078607 inhibits the progression of ovarian cancer via regulating the miR-32-5p/SIK1 network
The negative regulation of circ_0078607 on OC progression could be reversed by SIK1 silencing. HEY and ES-2 cells were transfected with NC, oe-circ_0078607, oe-circ_0078607 + si-NC or oe-circ_0078607 + si-SIK1. A-B QRT-PCR and WB analysis were used to test the mRNA and protein expression of SIK1. The EDU positive cells, the number of colonies, the numbers of migrated and invaded cells, and the apoptosis rate of cells were determined using EDU staining (C), colony formation assay (D), transwell assay (E-F) and flow cytometry (G). H The protein expression levels of E-cadherin, N-cadherin, MMP9 and Bax were evaluated by WB analysis. *P < 0.05, **P < 0.01, ***P < 0.001