Fig. 7

miR-324-3p over-expression promotes apoptosis and inhibits viability, migration, invasion, and EMT of ovarian cancer cells by negatively regulating LY6K. A. After transfection of mimics and plasmids, qRT-PCR observed the relative expression of LY6K in SKOV3 and A2780 cells. B. After transfection of mimics and plasmids, flow cytometry determined cell apoptosis of SKOV3 and A2780 cells. C. Cell proliferation of SKOV3 and A2780 cells after transfection of mimics and plasmids was observed by the EdU assay. D. Transwell assays examined SKOV3 and A2780 cell migration and invasion. E. Western blot was used to identify the expression of E-cadherin, N-cadherin and vimentin in ovarian cancer cells. Data were presented as mean ± SD. **p < 0.01, vs. mimics NC + pcDNA-NC. ^##p < 0.01, vs. miR-223-3p mimics + pcDNA-NC and mimics NC + pcDNA-LY6K