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Fig. 1 | Journal of Ovarian Research

Fig. 1

From: Minimal residual disease detection by multicolor flow cytometry in cryopreserved ovarian tissue from leukemia patients

Fig. 1

MFC gating strategy applied to detect MRD in ovarian samples. The observed populations are indicated at the top of the dot plots. The first gate is used for debris exclusion using SSC and FCS light scatter (Morphology). The 7-AAD or FVS780 combined with SYTO13+ set the nucleated viable cells. CD45 enabled us to separate CD45+ leucocytes from other viable cells for MRD analysis. A Gating strategy with 7-AAD. B Gating strategy with FVS780. Data acquired with Diva software and analysed with Flowjo software

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