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Table 2 MRD results of ovarian cortical tissue obtained by MFC and RT-qPCR

From: Minimal residual disease detection by multicolor flow cytometry in cryopreserved ovarian tissue from leukemia patients

Patient no Type of leukemia Ovarian cortical tissue Ovarian cortical tissue xenografts
MFC RT-qPCR MFC
Viable events Positive events MRD level MRD level Bone marrow Blood Spleen Lymph nodes
1 B-ALL 449 438 0 Negative
 < 5 × 10–5
Negative
Undetectable
Negative
 < 4 × 10–5
Negative
 < 6 × 10–5
Negative
 < 4 × 10–5
Negative
 < 2 × 10–4
2 B-ALL 295 103 3 Negative
 < 7 × 10–5
Negative
 < 1.10–4
Negative
 < 4 × 10–5
Negative
 < 8 × 10–5
Negative
 < 4 × 10–5
Negative
 < 3 × 10–4
3 B-ALL 333 314 1 Negative
 < 6 × 10–5
NP Negative
 < 3 × 10–5
Negative
 < 4 × 10–5
Negative
 < 3 × 10–5
Negative
 < 5 × 10–5
4 B-ALL 336 425 0 Negative
 < 6 × 10–5
NP Negative
 < 3 × 10–5
Negative
 < 2 × 10–4
Negative
 < 3 × 10–5
Negative
 < 8 × 10–5
5 B-ALL 233 918 0 Negative
 < 9 × 10–5
NA Negative
 < 5 × 10–5
Negative
 < 2 × 10–5
Negative
 < 5 × 10–5
Negative
7 × 10–5
6 B-ALL 233 868 0 Negative
 < 9 × 10–5
NP Negative
 < 2 × 10–5
Negative
 < 5 × 10–5
Negative
 < 5 × 10–5
Negative
 < 8 × 10–5
7 T-ALL 272 007 1 Negative
 < 8 × 10–5
NA NP NP NP NP
8 T-ALL 1,360,814 335 Positive
3 × 10–4
NA Negative
 < 6 × 10–5
Negative
 < 1 × 10–4
Negative
 < 3 × 10–5
Negative
 < 1 × 10–4
9 AML 254 777 7 Negative
 < 8 × 10–5
Negative
Undetectable
NP NP NP NP
10 AML 331 675 1 Negative
 < 6 × 10–5
NP Negative
 < 3 × 10–5
Negative
 < 9 × 10–5
Negative
 < 4 × 10–5
Negative
 < 7 × 10–5
11 AML 385 545 69 Positive
2 × 10–4
NP Negative
 < 3 × 10–5
Negative
 < 8 × 105
Negative
 < 3 × 10–5
Negative
 < 1 × 10–4
12 AML 133 216 34 Positive
3 × 10–4
NP Negative
 < 2 × 10–5
Negative
 < 7 × 10–5
Negative
 < 4 × 10–5
Negative
 < 6 × 10–5
13 AML 231 193 85 Positive
4 × 10–4
NA NP NP NP NP
14 AML 267 702 75 Positive
3 × 10–4
NA NP NP NP NP
15 AML 55 391 0 Negative
 < 4 × 10–5
NP NP NP NP NP
  1. Data present the number of leukemia patients and type of leukemia. Results of MRD detection in ovarian cortical tissue and after grafting experiments by MFC and RT-qPCR when possible are also presented. MRD values were obtained by dividing the number of cells in the LAIP gate (positive events) by the total number of viable events analysed. To assess the maximum of sensitivity corresponding to the limit of detection that can be achieved for each MRD experiment, we divided the minimal number of cells to define a significant abnormal cell population (20 LAIP+ cells) by the number of viable events analysed in this experiment
  2. B-ALL B-cell acute lymphoblastic leukemia, T-ALL T-cell acute lymphoblastic leukemia, AML acute myeloid leukemia, MFC multicolor flow cytometry, RT-qPCR reverse transcription-quantitative polymerase chain reaction, NA not available, NP not performed