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Table 2 MRD results of ovarian cortical tissue obtained by MFC and RT-qPCR

From: Minimal residual disease detection by multicolor flow cytometry in cryopreserved ovarian tissue from leukemia patients

Patient no

Type of leukemia

Ovarian cortical tissue

Ovarian cortical tissue xenografts

MFC

RT-qPCR

MFC

Viable events

Positive events

MRD level

MRD level

Bone marrow

Blood

Spleen

Lymph nodes

1

B-ALL

449 438

0

Negative

 < 5 × 10–5

Negative

Undetectable

Negative

 < 4 × 10–5

Negative

 < 6 × 10–5

Negative

 < 4 × 10–5

Negative

 < 2 × 10–4

2

B-ALL

295 103

3

Negative

 < 7 × 10–5

Negative

 < 1.10–4

Negative

 < 4 × 10–5

Negative

 < 8 × 10–5

Negative

 < 4 × 10–5

Negative

 < 3 × 10–4

3

B-ALL

333 314

1

Negative

 < 6 × 10–5

NP

Negative

 < 3 × 10–5

Negative

 < 4 × 10–5

Negative

 < 3 × 10–5

Negative

 < 5 × 10–5

4

B-ALL

336 425

0

Negative

 < 6 × 10–5

NP

Negative

 < 3 × 10–5

Negative

 < 2 × 10–4

Negative

 < 3 × 10–5

Negative

 < 8 × 10–5

5

B-ALL

233 918

0

Negative

 < 9 × 10–5

NA

Negative

 < 5 × 10–5

Negative

 < 2 × 10–5

Negative

 < 5 × 10–5

Negative

7 × 10–5

6

B-ALL

233 868

0

Negative

 < 9 × 10–5

NP

Negative

 < 2 × 10–5

Negative

 < 5 × 10–5

Negative

 < 5 × 10–5

Negative

 < 8 × 10–5

7

T-ALL

272 007

1

Negative

 < 8 × 10–5

NA

NP

NP

NP

NP

8

T-ALL

1,360,814

335

Positive

3 × 10–4

NA

Negative

 < 6 × 10–5

Negative

 < 1 × 10–4

Negative

 < 3 × 10–5

Negative

 < 1 × 10–4

9

AML

254 777

7

Negative

 < 8 × 10–5

Negative

Undetectable

NP

NP

NP

NP

10

AML

331 675

1

Negative

 < 6 × 10–5

NP

Negative

 < 3 × 10–5

Negative

 < 9 × 10–5

Negative

 < 4 × 10–5

Negative

 < 7 × 10–5

11

AML

385 545

69

Positive

2 × 10–4

NP

Negative

 < 3 × 10–5

Negative

 < 8 × 105

Negative

 < 3 × 10–5

Negative

 < 1 × 10–4

12

AML

133 216

34

Positive

3 × 10–4

NP

Negative

 < 2 × 10–5

Negative

 < 7 × 10–5

Negative

 < 4 × 10–5

Negative

 < 6 × 10–5

13

AML

231 193

85

Positive

4 × 10–4

NA

NP

NP

NP

NP

14

AML

267 702

75

Positive

3 × 10–4

NA

NP

NP

NP

NP

15

AML

55 391

0

Negative

 < 4 × 10–5

NP

NP

NP

NP

NP

  1. Data present the number of leukemia patients and type of leukemia. Results of MRD detection in ovarian cortical tissue and after grafting experiments by MFC and RT-qPCR when possible are also presented. MRD values were obtained by dividing the number of cells in the LAIP gate (positive events) by the total number of viable events analysed. To assess the maximum of sensitivity corresponding to the limit of detection that can be achieved for each MRD experiment, we divided the minimal number of cells to define a significant abnormal cell population (20 LAIP+ cells) by the number of viable events analysed in this experiment
  2. B-ALL B-cell acute lymphoblastic leukemia, T-ALL T-cell acute lymphoblastic leukemia, AML acute myeloid leukemia, MFC multicolor flow cytometry, RT-qPCR reverse transcription-quantitative polymerase chain reaction, NA not available, NP not performed