Table 1 Primer sequences used for quantitative Real-Time PCR
Genes | Primer sequences (5′ 3') | Optimized annealing Temperature(C°) | Product Size (bp) |
|---|---|---|---|
Bax | F: GCTGCAGACATGCTGTGGATC R: TCACAGCCAGGAGAATCGCAC | 60 | 419 |
Bcl-2 | F: TTAGAGAGATGCGAGGAACCG R: GGGACAAGTAAACCTGGAAGAA | 60 | 158 |
Cyt c | F: CGGCTGCTGTGATTGTGAAT R: TGTCTTGTGTTTCCCGCCTT | 60 | 157 |
GAPDH | F: AACTTTGGCATTGTGGAAGG R: ACACATTGGGGGTAGGAACA | 60 | 223 |