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Fig. 3 | Journal of Ovarian Research

Fig. 3

From: High expression of TRIM24 predicts worse prognosis and promotes proliferation and metastasis of epithelial ovarian cancer

Fig. 3

TRIM24 promotes EOC cell proliferation in vitro. a and b Western blotting and qRT-PCR analysis of TRIM24 protein and mRNA expression in IOSE and a panel of EOC cell lines. c The transfection efficiency of SKOV3 and CaOV-3 cells was detected by fluorescence imaging (magnification, × 200). d qRT-PCR analysis of TRIM24 knockdown in SKOV3 and overexpression in CaOV-3 cells. e Representative images of in vitro colony formation assay of SKOV3 and CaOV-3 cells. f Quantification of the EOC colonies shown in e respectively. g The effect of TRIM24 on EOC cell proliferation was determined by CCK-8 assay. h Western blot showed that knockdown of TRIM24 in SKOV3 cells could inhibit the phosphorylation of Akt, while overexpression of TRIM24 in CaOV-3 cells could promote the phosphorylation of Akt

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