Fig. 3

CircCDK17 directly interacted with miR-1294 in CC cells. A and B Cytoplasmic and nuclear RNA analysis assay demonstrated the cell location of circCDK17, β-actin and U6 in C-33A and HeLa cells. C The binding sites between circCDK17 and miR-1294 were predicted by interactome online database. D and E Luciferase activity was detected by dual-luciferase reporter assay in C-33A and HeLa cells. F and G RNA pull-down assay was employed to illustrate circCDK17 was directly associated with miR-1294 in C-33A and HeLa cells. H and I MiR-1294 expression was detected by qRT-PCR in CC tissues, normal cervical tissues and Ect1/E6E7, C-33A and HeLa cells. J CircCDK17 expression was determined by qRT-PCR in C-33A and HeLa cells transfected with circCDK17 or vector. K QRT-PCR was performed to illustrate the influence of circCDK17 overexpression on miR-1294 expression in C-33A and HeLa cells. *P < 0.05