Fig. 4

CircCDK17 regulated biological behavior of CC by binding to miR-1294. A The effects between circCDK17 repression and miR-1294 inhibitor on miR-1294 expression were investigated by qRT-PCR in C-33A and HeLa cells. B and C The impacts between circCDK17 knockdown and miR-1294 inhibitor on the proliferation of C-33A and HeLa cells were disclosed by CCK-8 assay. D and E Transwell assay was performed to illustrate the influences between circCDK17 repression and miR-1294 inhibitor on the migration and invasion of C-33A and HeLa cells. F Flow cytometry analysis was employed to elucidate the impacts between circCDK17 knockdown and miR-1294 inhibitor on cell apoptosis in C-33A and HeLa cells. G Lactate assay kit was carried out to demonstrate the effects between circCDK17 silencing and miR-1294 inhibitor on lactate production in C-33A and HeLa cells. H Glucose assay kit was used to determine the impacts between circCDK17 knockdown and miR-1294 inhibitor on glucose uptake in C-33A and HeLa cells. I ATP detection kit was conducted to present the effects between circCDK17 silencing and miR-1294 inhibitor on ATP level in C-33A and HeLa cells. J and K Western blot was conducted to illustrate the effects between circCDK17 downregulation and miR-1294 repression on the protein expression levels of GLUT1 and HK2 in C-33A and HeLa cells. *P < 0.05