Fig. 5

MiR-1294 bound to YWHAZ in CC cells. A The binding sites between miR-1294 and YWHAZ were predicted by targetscan online database. B and C Luciferase activities were detected by dual-luciferase reporter assay in C-33A and HeLa cells. D-F QRT-PCR and western blot analysis were employed to detect the mRNA and protein expression levels of YWHAZ, respectively, in CC tissues, normal cervical tissues or Ect1/E6E7, C-33A and HeLa cells. G The transfection efficiency of miR-1294 mimic and inhibitor was determined by qRT-PCR in C-33A and HeLa cells. H and I Western blot analysis was performed to present the effects of miR-1294 mimic and inhibitor on YWHAZ protein expression in C-33A and HeLa cells. (J) The impacts between circCDK17 knockdown and miR-1294 inhibitor on YWHAZ protein expression were illustrated by western blot in C-33A and HeLa cells. *P < 0.05