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Fig. 6 | Journal of Ovarian Research

Fig. 6

From: CircCDK17 knockdown inhibits tumor progression and cell glycolysis by downregulaing YWHAZ expression through sponging miR-1294 in cervical cancer

Fig. 6

MiR-1294 mimic inhibited CC process by binding to YWHAZ. A Western blot was employed to illustrate the effects between miR-1294 mimic and YWHAZ on YWHAZ protein expression in C-33A and HeLa cells. B and C The impacts between miR-1294 and YWHAZ on the proliferation of C-33A and HeLa cells were investigated by CCK-8 assay. D and E The influences between miR-1294 mimic and enforced YWHAZ expression on the migration and invasion of C-33A and HeLa cells were demonstrated by transwell assay. F Flow cytometry analysis was performed to elucidate the impacts between miR-1294 and YWHAZ on cell apoptosis in C-33A and HeLa cells. G Lactate assay kit was conducted to demonstrate the effects between miR-1294 and YWHAZ on lactate production in C-33A and HeLa cells. H Glucose assay kit was used to determine the impacts between miR-1294 mimic and YWHAZ on glucose uptake in C-33A and HeLa cells. I The effects between miR-1294 and ectopic YWHAZ expression on ATP level were presented by ATP detection kit in C-33A and HeLa cells. J and K Western blot was carried out to elaborate the effects between miR-1294 and circCDK17 on the protein expression levels of GLUT1 and HK2 in C-33A and HeLa cells. *P < 0.05

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